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fncas12a protein  (Beyotime)


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    Beyotime fncas12a protein
    Fncas12a Protein, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fncas12a+protein/FnCas12a/pmc13126157-165-1-9
    Average 99 stars, based on 12 article reviews
    fncas12a protein - by Bioz Stars, 2026-10
    99/100 stars

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    Purification:

    Article Title: RPA assay coupled with CRISPR/Cas12a system for the detection of seven Eimeria species in chicken fecal samples.
    Article Snippet: Chicken coccidiosis is one of the most common and economically important diseases in the global poultry industry, and it is caused by at least one of the seven Eimeria species.. A simple and reliable way to distinguish Eimeria species in infected chicken is critical for the surveillance, control, and eradication of chicken coccidiosis.. In this study, a recombinase polymerase amplification (RPA) assay coupled with the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system (RPA–CRISPR/Cas12a) was developed for the detection of Eimeria species in chicken fecal samples.

    Article Title: FnCas12a/crRNA-Mediated Genome Editing in Eimeria tenella
    Article Snippet: The coding sequence of FnCas12a is obtained from plasmid #69976 (Addgene), and the plasmid sequence for the expression of FnCas12a in E. coli is provided in . .. The FnCas12a protein was purified using the BeyoGoldTM His-tag Purification Resin (P2210, Beyotime Biotechnology) according to the manufacturer’s instructions. ..



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    crRNA-Free DNase Activities of Cas12a Orthologs toward Circular DNA (A) Representative in vitro cleavage of circular phage M13mp18 ssDNA by AsCas12a and LbCas12a in the presence of various divalent metal ions. <t>FnCas12a</t> was used as a positive group. Control (Ctrl), M13mp18 ssDNA alone. (B) Metal ion concentration dependence for cleavage of M13mp18 ssDNA substrates by AsCas12a and LbCas12a. Different concentrations of Mg 2+ or Mn 2+ ranging from 0.5 to 10 mM were used. Ctrl, M13mp18 ssDNA alone. (C) Representative in vitro cleavage of circular plasmid pUC19 dsDNA by AsCas12a and LbCas12a in the presence of various divalent metal ions. FnCas12a was used as a positive group. Ctrl, pUC19 dsDNA alone. (D) Metal ion concentration dependence for cleavage of pUC19 dsDNA substrates by AsCas12a and LbCas12a. Different concentrations of Mg 2+ or Mn 2+ ranging from 0.5 to 10 mM were used. Ctrl, pUC19 dsDNA alone. Positions of M13mp18, supercoiled (SC, green arrow), nicked (N, red arrow), and linear (L, red arrow) DNA were indicated.
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    crRNA-Free DNase Activities of Cas12a Orthologs toward Circular DNA (A) Representative in vitro cleavage of circular phage M13mp18 ssDNA by AsCas12a and LbCas12a in the presence of various divalent metal ions. FnCas12a was used as a positive group. Control (Ctrl), M13mp18 ssDNA alone. (B) Metal ion concentration dependence for cleavage of M13mp18 ssDNA substrates by AsCas12a and LbCas12a. Different concentrations of Mg 2+ or Mn 2+ ranging from 0.5 to 10 mM were used. Ctrl, M13mp18 ssDNA alone. (C) Representative in vitro cleavage of circular plasmid pUC19 dsDNA by AsCas12a and LbCas12a in the presence of various divalent metal ions. FnCas12a was used as a positive group. Ctrl, pUC19 dsDNA alone. (D) Metal ion concentration dependence for cleavage of pUC19 dsDNA substrates by AsCas12a and LbCas12a. Different concentrations of Mg 2+ or Mn 2+ ranging from 0.5 to 10 mM were used. Ctrl, pUC19 dsDNA alone. Positions of M13mp18, supercoiled (SC, green arrow), nicked (N, red arrow), and linear (L, red arrow) DNA were indicated.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: CRISPR-Cas12a Possesses Unconventional DNase Activity that Can Be Inactivated by Synthetic Oligonucleotides

    doi: 10.1016/j.omtn.2019.12.038

    Figure Lengend Snippet: crRNA-Free DNase Activities of Cas12a Orthologs toward Circular DNA (A) Representative in vitro cleavage of circular phage M13mp18 ssDNA by AsCas12a and LbCas12a in the presence of various divalent metal ions. FnCas12a was used as a positive group. Control (Ctrl), M13mp18 ssDNA alone. (B) Metal ion concentration dependence for cleavage of M13mp18 ssDNA substrates by AsCas12a and LbCas12a. Different concentrations of Mg 2+ or Mn 2+ ranging from 0.5 to 10 mM were used. Ctrl, M13mp18 ssDNA alone. (C) Representative in vitro cleavage of circular plasmid pUC19 dsDNA by AsCas12a and LbCas12a in the presence of various divalent metal ions. FnCas12a was used as a positive group. Ctrl, pUC19 dsDNA alone. (D) Metal ion concentration dependence for cleavage of pUC19 dsDNA substrates by AsCas12a and LbCas12a. Different concentrations of Mg 2+ or Mn 2+ ranging from 0.5 to 10 mM were used. Ctrl, pUC19 dsDNA alone. Positions of M13mp18, supercoiled (SC, green arrow), nicked (N, red arrow), and linear (L, red arrow) DNA were indicated.

    Article Snippet: FnCas12a protein is from Applied Biological Materials.

    Techniques: In Vitro, Control, Concentration Assay, Plasmid Preparation

    Inhibitory Effects of Anti-Cas12a psDNA on Cas12a-Mediated DNase Activity toward Circular DNA (A) Dose-dependent inhibitory effects of anti-Cas12a psDNA on Mg 2+ -promoted AsCas12a activity. Control (Ctrl), M13mp18 ssDNA alone. ssDNA1 was parallelly used to rule out the possibility of oligonucleotides interference. The concentration of AsCas12a was 200 nM. (B–D) The effects of anti-Cas12a psDNA on metal-dependent ssDNase activity of Cas12a orthologs (AsCas12a, LbCas12a, and FnCas12a) on circular ssDNA M13mp18 (B), ΦX174 (C), and dsDNA pUC19 (D). The concentration of anti-Cas12a psDNA was 200 nM. Ctrl, M13mp18 ssDNA alone. The vertical dotted line indicates the border between two separate gels. Ctrl, pUC19 dsDNA alone. Positions of M13, ΦX174, supercoiled (SC), nicked (N), and linear (L) DNA were indicated.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: CRISPR-Cas12a Possesses Unconventional DNase Activity that Can Be Inactivated by Synthetic Oligonucleotides

    doi: 10.1016/j.omtn.2019.12.038

    Figure Lengend Snippet: Inhibitory Effects of Anti-Cas12a psDNA on Cas12a-Mediated DNase Activity toward Circular DNA (A) Dose-dependent inhibitory effects of anti-Cas12a psDNA on Mg 2+ -promoted AsCas12a activity. Control (Ctrl), M13mp18 ssDNA alone. ssDNA1 was parallelly used to rule out the possibility of oligonucleotides interference. The concentration of AsCas12a was 200 nM. (B–D) The effects of anti-Cas12a psDNA on metal-dependent ssDNase activity of Cas12a orthologs (AsCas12a, LbCas12a, and FnCas12a) on circular ssDNA M13mp18 (B), ΦX174 (C), and dsDNA pUC19 (D). The concentration of anti-Cas12a psDNA was 200 nM. Ctrl, M13mp18 ssDNA alone. The vertical dotted line indicates the border between two separate gels. Ctrl, pUC19 dsDNA alone. Positions of M13, ΦX174, supercoiled (SC), nicked (N), and linear (L) DNA were indicated.

    Article Snippet: FnCas12a protein is from Applied Biological Materials.

    Techniques: Activity Assay, Control, Concentration Assay

    Inhibitory Effects of Anti-Cas12a psDNA on Cas12a-Mediated DNase Activity toward Linear DNA (A–C) The effects of anti-Cas12a psDNA on metal-dependent ssDNase activity of Cas12a orthologs (AsCas12a, LbCas12a, and FnCas12a) toward linear ssDNA (A), dsDNA (B), and linearized pUC19 (C).

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: CRISPR-Cas12a Possesses Unconventional DNase Activity that Can Be Inactivated by Synthetic Oligonucleotides

    doi: 10.1016/j.omtn.2019.12.038

    Figure Lengend Snippet: Inhibitory Effects of Anti-Cas12a psDNA on Cas12a-Mediated DNase Activity toward Linear DNA (A–C) The effects of anti-Cas12a psDNA on metal-dependent ssDNase activity of Cas12a orthologs (AsCas12a, LbCas12a, and FnCas12a) toward linear ssDNA (A), dsDNA (B), and linearized pUC19 (C).

    Article Snippet: FnCas12a protein is from Applied Biological Materials.

    Techniques: Activity Assay